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NLRP3 Antibody santa Cruz

High-Quality antibodies for Vaccine Development. Fast Delivery. Welcome Enquiry! Browse our Antibodies/Antigenes. View Specifications. Prices & more. Buy online Cryopyrin/NALP3/NLRP3 Antibody (6F12) is a monoclonal anti-NLRP3 antibody that detects human Cryopyrin by WB, IP and ELISA. Cited in 20 publications. Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market Anti-NLRP3 Antibody Products from Santa Cruz Biotechnology, Inc. Anti-NLRP3 antibodies are offered by a number of suppliers. This target gene encodes the protein 'NLR family pyrin domain containing 3' in humans and may also be known as Cryopyrin, Fcu, NALP3, AGTAVPRL, AII, and NACHT, LRR and PYD domains-containing protein 3 SANTA CRUZ BIOTECHNOLOGY, INC. Cryopyrin (6F12): sc-134306 Genetic locus: NLRP3 (human) mapping to 1q44. SOURCE Cryopyrin (6F12) is a mouse monoclonal antibody raised against a recombinant protein corresponding to a region near the N-terminus of Cryopyrin of human origin

A gain-of-function NLRP3 3'-UTR polymorphism causes miR-146a-mediated suppression of NLRP3 expression and confers protection against sepsis progression. View PDF In Scientific Reports on 25 June 2021 by Lu, F., Chen, H., et al. Santa Cruz Biotechnology NLRP3 antibody (Santa Cruz, sc-134306) was used in western blot on human samples at 1:1000 (fig 1). Biomed Res Int (2015) ncbi mouse monoclonal (6F12

Cryopyrin Antibody (H-66) is a rabbit polyclonal IgG; 200 µg/ml. epitope corresponding to amino acids 25-90 mapping near the N-terminus of Cryopyrin of human origin. Discontinued polyclonal antibody The sections were then incubated with primary antibody against CD68 (Abcam), NLRP3 (AdipoGen), caspase‐1 (Santa Cruz), and MMP‐9 (Santa Cruz) at 4°C overnight; washed with Perm/Wash; and incubated with secondary antibody conjugated to an Alexa Fluor dye such as Alexa Fluor‐488, ‐568, or ‐647 (Thermo Fisher Scientific) The platelets were then incubated with goat anti-NLRP3 antibody (Novus, NB100-41104, 1:100), mouse anti-ASC antibody (Santa Cruz, sc-271054, 1:100), and rabbit anti-CD41 antibody (Abcam, ab134131, 1:300) at 4°C overnight, followed by incubation with 1:200 dilutions of relative secondary antibody for 1 h SANTA CRUZ BIOTECHNOLOGY, INC. ASC (F-9): sc-271054 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND Caspase-associated recruitment domains (CARDs) mediate the interactio

Nlrp3 Antibody | Bioz | Ratings For Life-Science Research

Santa Cruz Biotechnology - Leader In Biomedical Product

  1. (sc-66846) Cryopyrin Antibody (H-66) This product has been discontinued by Santa Cruz Biotechnology, but remains here on CiteAb for record purposes. Antibody inf
  2. (USA). Polyclonal anti-pro-IL-1β, anti-NLRP3 antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, USA). Cytokine detection by ELISA FLS were plated at 2.0 × 104 cells/well in a 24-well plate. When they reached subconfluency, the medium was re-moved, and fresh medium was applied along with MSU (1, 10, 50, 100, 200, 500 ug/ml)
  3. at 37 °C
  4. NLRP3 antibody (1:200 dilution, Santa Cruz Biotechnology, Santa Cruz, CA), anti-ASC antibody (1:200 dilution, Santa Cruz Biotechnology), and anti-caspase-1 antibody (1:200 dilution, Santa Cruz Biotechnology) at 4 °C overnight, followed by incubation with goat anti-rabbit biotinylated antibody (ZSGB-Bio, Beijing, China) at room temperature

Antibody Products for Vaccine - Development & Researc

  1. Western blot of NLRP3 Antibody in MDA-MB231 cell line lysates (35 ug/lane). NLRP3 (arrow) was detected using the purified antibody. NLRP3 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human lymph tissue followed by peroxidase-conjugated secondary antibody and DAB staining. quantity: price: to the supplie
  2. on ice in lysis buffer containing the.
  3. The 5-μm serial sections were reacted with affinity-purified anti-NLRP3 polyclonal antibody (Abcam). The slides were washed in phosphate-buffered saline (PBS) and subsequently incubated with the second antibody, HRP-conjugated goat anti-rabbit IgG (Santa Cruz Biotechnology) for 1 h at room temperature

Anti-Cryopyrin/NALP3/NLRP3 Antibody (6F12) SCBT - Santa

Anti-NLRP3 Antibody Products from Santa Cruz Biotechnology

Protein complexes were washed five times with RIPA buffer, resuspended in 2× loading buffer, and heated at 95 °C for 10 min before western blot analysis by using the following antibodies: rabbit anti-ASC (1:1000, Santa Cruz), rabbit anti-NALP1 (1:200: Abcam), rabbit anti-NLRP3 (1:200, Santa Cruz), and rabbit anti-caspase-1 (1:200: Santa Cruz) The NLRP3 inflammasome and IL-1β have recently been linked to the severity of uropathogenic Escherichia coli (UPEC)-mediated urinary tract infection (UTI). However, not much is known about the.

Anti-IL-1β antibody (Abcam) and anti-IL-18 antibody (Santa Cruz Biotechnology, Dallas, TX) were used as primary antibodies in this study. After incubation with primary antibody overnight, the sections were washed in PBS and incubated with biotinylated IgG (1:200) for 1 h and then with streptavidin-HRP for 30 min at room temperature minutes at roo m tempe rature and incubated with primary NLRP3 antibody (1:200, Santa Cruz Biote chnology Cat# sc- 66 846, RRID: AB_2152446), caspase-1 antibody (1:200 To detect NLRP3 and ASC, the blots were probed with 1∶1000 rabbit anti-human NLRP3 antibody (Sigma) and mouse anti-human ASC antibody (Santa Cruz Biotechnology), respectively. The signals on the blots were visualized using the enhanced chemiluminescence system (Millipore) antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) diluted at 1:800, rabbit anti-NLRP3 polyclonal antibody (Abcam, Cambridge, MA, USA) with a dilution of 1:1,000 or anti-GAPDH (glyceraldehyde phosphate dehydrogenase) antibody with a dilution of 1:1,000 (Santa Cruz Biotechnology) as a loading control. After washin

Secondary antibodies were identical to those mentioned above. In addition, acetone-fixed cryostat sections (4 μm) of the synovia biopsies were washed with PBS and blocked for 1 h with 5% bovine serum albumin (BSA) in PBS. AlexaFluor A594-conjugated anti-CD14 antibody (Santa Cruz Inc.) was used to label monocytes/macrophages Polyclonal anti-pro-IL-1β, anti-NLRP3 antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, USA). Cytokine detection by ELISA. FLS were plated at 2.0 × 10 4 cells/well in a 24-well plate. When they reached subconfluency, the medium was removed, and fresh medium was applied along with MSU (1, 10, 50, 100, 200, 500 ug/ml). The. The sc-22166 antibody from Santa Cruz against cleaved caspase-1 p10 is a goat polyclonal antibody raised against a short amino acid sequence containing the neoepitope at Gly315 of caspase-1 of mouse origin. Proteolytic cleavage of the precursor caspase-1 at glycine residue 317 in humans and 315 in mouse generates the functional caspase-1. NLRP3 over-activation is associated with a breakdown of enteric-immune balance related to IBS-D. (sc-52746 Santa Cruz Biotechnology, Dallas, TX, USA 1:100 in PBS, v/v), anti-IL-1 (sc-32294 Santa Cruz incubated with a secondary antibody (Santa Cruz Biotechnology, Dallas, TX, USA) for 1 h. The reactio The Nlrp3 inflammasome promotes myocardial dysfunction frozen sections using rat anti-CD3 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Terminal deoxynucleotidyl transferasedUTP nick end labeling (TUNEL) stain was performed on paraffin-embedde

(sc-134306) Anti-Cryopyrin/NALP3/NLRP3 Antibody (6F12

The membrane was blocked with 5% nonfat milk and incubated with anti-NLRP3 antibody, ASC antibody, anti-caspase-1 antibody, and anti-IL-1β antibody (Santa Cruz Biotechnology, Inc., Dallas, TX, USA). Blots were developed using enhanced chemiluminescent substrate (Thermo Fischer Scientific Pierce, IL, USA). Monolayer Wound Healing Assa NLRP3 or RIG-I expression in cardiac myocytes was assessed by Western blot analysis with NLRP3 antibody (Santa Cruz Biotechnology) or RIG-I antibody (Cell Signaling Technology), respectively, and quantified using ImageJ software. ELISA for IL-1β serum (Sigma, USA), then incubated with anti- IL-1β antibody for 24 hours (Abcam, USA). A fluorescein second antibody (Santa Cruz Biotechnology, USA) was then incubated for one hour at 37°C. Vectashield Mounting Medium with DAPI (VECTOR, USA) was used for nuclear staining. Images were acquired by fluorescence microscope (Life Technologies, USA) Anti-NLRP3, anti-ASC, anti-Caspase-1, and anti-GAPDH were purchased from Santa Cruz Biotechnology, Inc. The lung tissues of rats in each group were cut into small fragments, RIPA buffer (100 uL of lysis buffer was added for every 20 mg of tissue) was added, and then homogenized using a glass homogenizer until fully decomposed with ASC primary antibody (Santa Cruz Biotechnology, sc-33958, 1:100 diluted) overnight, followed by Alexa fluor-594 conjugated anti-goat antibody (Jackson Immunoresearch, 705-585-003, 1:1000) for one h. ASC speck images were acquired with LSM 880 Confocal microscopy (Zeiss, Germany). ASC speck positive cells were counted by ImageJ (Nationa

NLRP3 antibody antibody review based on formal publication

HEK293T cells (c) or A549 cells (d) were co-transfected with Flag-NLRP3 and HA-SARS-CoV-2-N. Cell lysates were immunoprecipitated using anti-Flag antibody or anti-HA antibody, and analyzed using. antibody (Santa Cruz; 1 : 300 dilution), anti-p65 antibody (A ffi nity; 1 : 1000 dilution), anti- β -actin antibody (Santa Cruz; 1 : 1000 dilution), and anti-Histone H3 antibody Blots were incubated with anti‐IL‐1 antibody (R&D, AF‐201‐NA), anti‐CASP1‐p20 antibody (Adipogen, AG‐20B‐0048‐C100), anti‐NLRP3 antibody (Adipogen, AG‐20B‐0014‐C100), anti‐ASC antibody (Santa Cruz Biotechnology, sc‐22514‐R), and anti‐CASP4 antibody (Santa Cruz Biotechnology, sc‐56056) for 72 h as primary and. NLRP3, or ASC antibody (Santa Cruz Biotechnology), and then incubated in the mixture of FITC anti-rabbit IgG antibody (Cell Signaling Technology) and anti-mouse CD31(PECAM-1)-PE (eBioscience). After 5 min of incubation in 4′, 6-diamidino-2-phenylindole (DAPI), the sections were photographed with a fluorescence mi-croscope

Cryopyrin Antibody (H-66) SCBT - Santa Cruz Biotechnolog

NLRP3 inflammasome is a protein complex composed of NOD-like receptor protein 3 (NLRP3), apoptosis-associated speck-like protein containing a CARD (ASC), and caspase-1. Fully assembled NLRP3 inflammasome cleaves pro-form caspase-1(p45) into active caspase-1(p20), and the activ beta receptor (1:50, Santa Cruz), goat anti-ionized calcium-binding adapter molecule 1 (Iba 1, 1:200, Abcam), and rabbit anti-MPO (1:100, Abcam) at 4 °C overnight. After being washed three times with PBS, the sections were incubated with appropriate fluorescence-conjugated secondary antibodies (1:300, Jackson Immu brane was probed with primary antibody for IL-1β (1:1000, Abcam), caspase-1 (1:1000, Santa Cruz) or NLRP3 (1:500, Santa Cruz), and then incubated with the peroxidase-conju-gated secondary antibody (1:3000, Santa Cruz). Protein bands were detected by ECL (Pierce) and visualized by gel imagin CD3 staining was performed on frozen sections using rat anti-CD3 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Terminal deoxynucleotidyl transferasedUTP nick end labeling (TUNEL) stain was performed on paraffin-embedded sections using the in situ cell death detection kit according to the manufacturer's protocol (Roche, San Francisco.

Targeting the NLRP3 Inflammasome With Inhibitor MCC950

Detection of NLRP3 protein level by Western blotting Western blotting analysis was performed, as previously described [22]. Membranes were incubated with anti-NLRP3 antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and the secondary antibody was linked to horseradish peroxidase (1:2000; Invitrogen, Carlsbad, CA, USA) Specimens were then incubated in a humidified chamber for 2 hours at room temperature with an anti-NLRP3 antibody (Adipogen, San Diego, CA, USA), anti-ASC antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) or anti-cleaved caspase-1 antibody (Santa Cruz Biotechnology) The membranes were then blocked with 5% skim milk for 2 h at 37 °C and incubated overnight at 4 °C with the following antibodies: anti-NLRP3 antibody (Santa Cruz Biotechnology) at 1:200 dilution, anti-ASC antibody (Santa Cruz Biotechnology) at 1:200 dilution, anti-caspase-1 antibody (Proteintech, Wuhan, China) at 1:500 dilution, antibodies to. NLRP3 inflammasome gene expression was analyzed in respiratory epithelial cells and alveolar macrophages obtained from ventilated patients (n = 40). To detect caspase-1 a rabbit polyclonal antimouse caspase-1 p10 antibody (Santa Cruz Biotechnology, Santa Cruz, CA). I/R induced the formation of nucleotide-binding domain leucine-rich repeat containing family pyrin domain containing 3 (NLRP3) and absent in melanoma 2 (AIM2) inflammasomes and the subsequent serum release of interleukin 1β. Pannexin-1 inhibitor and anti-cathepsin B antibody attenuated I/R-induced inflammasome activation and hepatic injury

An amount of 100 µl of 3D5 monoclonal antibody (Santa Cruz Biotechnology, Inc., sc-47696) with a final concentration of 1 µg/ml was added to each well of the 96-well plate, incubated at 37°C for 2 h for coating, and washed with PBST after culturing overnight at 4°C The NLRP3 inflammasome is a prominent and early mediator of inflammatory responses in myocardial injury . NLRP3 inflammasome activation serves an important role in high glucose-induced H9c2 cell toxicity . Therefore, whether propofol prevented NLRP3 inflammasome activation in LPS-induced cardiomyocytes was investigated Proteins were transferred onto nitrocellulose membranes and incubated with p-MK2 (Thr334), MK2, p-p38α, p38α antibody (1:1,000; Cell Signaling Technologies), NLRP3 antibody (AdipoGen), pro-caspase-1, or β-actin antibody (Santa Cruz Biotechnology, Inc.) overnight at 4°C followed by incubation for 1 h with secondary goat anti-mouse IRDye. The membranes were blocked with 5% nonfat skim milk in TBS containing 0.1% Tween 20 (TBST) for 1 h and incubated with primary antibodies against IL-1β, NLRP1, NLRP3, NLRC4, AIM2, cleaved Casp-1 (Asp297), Casp-1, ASC, P2X7 receptor (P2X7R) (all from Cell Signaling Technology, Danvers, MA, USA), or α-Tubulin (Santa Cruz Biotechnology, Santa.

Then, cells were incubated with mouse anti-GCN5L1 antibody (Santa Cruz, sc515444), rabbit anti-E-cadherin antibody (Proteintech, 20874-1-AP), and rabbit anti-αSMA antibody (Proteintech, 55135-1-AP) overnight at 4°C. Secondary antibody (Alexa Fluor 488 Goat Anti-Rabbit IgG H&L, ab150077, 1 : 500) was used to stain the cells, and DAPI nuclear. The NLRP3 inflammasome was co-immunoprecipitated using Dynabeads Co-Immunoprecipitation Kit (Life Technologies Corporation, Grand Island, New York) according to the manufacturer's instructions. Anti-ASC antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, California) or control IgG (Santa Cruz Biotechnology, Inc.) was incubated with Dyna Results: F.tularensis represses AIM2 and NLRP3 inflammasomes in a FTL_0325-dependent fashion. Conclusion: Repression of inflammasome by F.tularensis results in fulminate infection. bovine-anti-rabbit monoclonal IgG-HRP antibody (Santa-Cruz, sc-2385), and for both IL-1 and IL-18, a bovine-anti

Frontiers Activation of Platelet NLRP3 Inflammasome in

  1. NLRP3 acts as a sensor of cellular changes induced by certain danger signals (21). primary antibodies used were a rabbit anti-ASC antibody (Santa Cruz Biotechnology), a rabbit anti-calmodulin anti-body (Abcam, Cambridge, UK), or a goat anti-human IL-1 antibody (R&D Systems). The secondary antibodies used wer
  2. Anti-LHX6 Antibody Products from Santa Cruz Biotechnology, Inc. Anti-LHX6 antibodies are offered by a number of suppliers. This target gene encodes the protein 'LIM homeobox 6' in humans and may also be known as Lhx6.1, LIM/homeobox protein Lhx6, and LIM homeodomain protein 6.1. Structurally, the protein is reported to be 40 kilodaltons in mass
  3. For cleaved caspase-3, NLRP3, and p65 staining, tissue sections or cells were subjected to incubation with anti-cleaved caspase-3 (Affinity, China), anti-NLRP3 (ABclonal), and anti-p65 (Affinity) antibodies (1 : 100 dilution) at 4°C overnight, followed by incubation with Cy3-conjugated goat anti-rabbit IgG (1 : 200 dilution) at room.
  4. The siRNA pools targeting human FFAR4 (sc-60737), GPR84 (sc-60751), or a control (sc-37007, Santa Cruz Biotechnology) were prepared with 2 µl HD Transfection Reagent (Promega) and used at a concentration of 40 nM to treat differentiated THP-1 cells overnight. One day later the cells were primed with LPS and treated with ATP as above
  5. The membrane was probed with primary antibody for IL-1[beta] (1:1000, Abcam), caspase-1 (1:1000, Santa Cruz) or NLRP3 (1:500, Santa Cruz), and then incubated with the peroxidase-conjugated secondary antibody (1:3000, Santa Cruz). Protein bands were detected by ECL (Pierce) and visualized by gel imaging system (Bio-Rad)
  6. MM is characterized by β2m abundance. The role of β2m remains unknown. Hofbauer et al. show that MM-associated macrophages take up β2m. Phagocytosed β2m forms β-fibrils and leads to lysosomal rupture and inflammasome activation. They reveal β2m as a promoter of inflammation, which plays a role in MM initiation and progression

(sc-66846) Cryopyrin Antibody (H-66) - Santa Cruz

Endoplasmic reticulum stress may activate NLRP3

  1. , and removed by centrifugation.
  2. Paraquat (PQ) can cause multiorgan failure including acute kidney injury (AKI). Our prior study showed that Toll-interacting protein (TOLLIP) protected against PQ-induced acute lung injury. However, the role of TOLLIP in PQ-induced AKI remains undefined. This study was aimed at understanding the role and mechanism of TOLLIP in AKI. Six-eight-week-old male Wistar rats were intraperitoneally.
  3. The membrane was preblocked with 5% nonfat milk and 0.1% Tween-20 in Tris-buffered saline and was incubated overnight with rabbit anti-SMP30 polyclonal antibody (pAb) (Santa Cruz Biotechnology), rabbit anti-eNOS pAb (Santa Cruz Biotechnology), rabbit anti-NLRP3 pAb (Novus Biologicals), mouse anti-ASC monoclonal antibody (Santa Cruz.

Video: NLRP3 antibody 14 (5 knockout-validated) products in

NLRX1 modulates differentially NLRP3 inflammasome activation 13 Anti-TOPO-I (sc-5342) was purchased from Santa Cruz. Gel electrophoresis 14 reagents were from Bio-Rad. 15 16 . 2.2. Cells and bacterial Culture 24 IgG antibody conjugated with red fluorescent Alexa Fluor 546 dye (Invitrogen) for. For NLRP3 and pTyr detection in immunoprecipitates, mouse anti-NLRP3 (Enzo Life Sciences; Cryo-1, 804-880 or Nalpy-3a, 804-881) and mouse anti-pTyr (CST; P-Tyr-100, 9411) antibodies were used. For detection of PTPN22, rabbit anti-PTPN22 antibody (Santa Cruz Biotechnology Inc.; H-253, sc-134782) was used. RNA isolation, RT-PCR, and quantitative PCR The membranes were blocked in Tris-buffered saline (20 mM Tris·HCl at pH 7.5 and 150 mM NaCl) with 0.1% Tween 20 (TBS-T) containing 5% skim milk and then incubated with anti-NF-B, antiphosphor-NF-B antibody, anti-cleaved casp-3 antibody (Cell Signaling Technology, Beverly, MA), or anti-caspase-1 p10 antibody (Santa Cruz Biotechnology, Dallas, TX) antibody (Cat. #sc-293150) were from Santa Cruz Biotechnology (California, United States). stained by antibodies against NLRP3 according to the. manufacturer.

anti-mouse protein antibody (Santa Cruz Biotechnology, 1:600) at 37 C for 1 hour and visualized using PV-6001 Poly-mer Detection System (Golden Bridge International Inc.) fol-lowing the manufacturer's instructions. Subsequently, slides were incubated with 30-diaminobenzidine tetrahydrochloride-H 2 IL-1β secretion by Pam3CSK4-primed C57BL/6 BMDMs treated with indicated doses of α-IFNβ antibody (Santa Cruz) or 10 μg/ml of isotype antibody and stimulated with EHEC or polydAdT for 16 hr. Data are presented as the mean ± SEM of one experiment representative of two experiments

NLRP3-inflammasome activation is associated with

NLRP3 protein level was inversely correlated with E-cadherin whereas it positively was correlated with IL-β, active NF-κB, N cadherin, vimentin, and MMP 9. antibody (Santa Cruz, sc-130348), mouse anti-IL1β antibody (Santa Cruz, sc-12742), rabbit antiNLRP To detect NLRP3 and ASC, the blots were probed with 1:1000 rabbit anti-human NLRP3 antibody (Sigma) and mouse anti-human ASC antibody (Santa Cruz Biotechnology), respectively. The signals on the blots were visualized using the enhanced chemiluminescence system (Millipore) The membranes were blocked with 5% dry milk in TBST and incubated with rabbit poly- clonal anti-NLRP3, anti-ER β , anti-Caspase-1, anti-IL- 1 β , anti-IL-18 (ABclonal, USA), anti-GAPDH (Santa Cruz, USA) or rabbit monoclonal anti-ER α (Cell Signaling Technology, USA) at 4°C overnight. After being washed, the bound antibodies were detected.

Cholesterol-dependent cytolysins induce rapid release of mature IL-1β from murine macrophages in a NLRP3 inflammasome and cathepsin B-dependent manner Frederick Cancer Research and Development Center, Frederick, MD, USA) and goat anti-mouse IgG-HRP secondary antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) using the SNAP i.d. NLRP3 may play a role independently of its canonical role as a component of inflammasomes. 20 . 21 . 3 1 . and antibody (Santa Cruz ERK1 9 Biotechnology, Santa Cruz, CA). In some experiments, the intensity of each band was quantified 10 TNFAIP3 and DEPTOR together rapidly promoted autophagy after LPS treatment to prevent NLRP3 inflammasome formation. USA). Antibodies used were: anti-TNFAIP3 mouse antibody (Santa Cruz Biotechnology, sc-166692), anti-TNFAIP3 goat antibody (Santa Cruz Biotechnology, sc-32525; this antibody is no longer available from this source), anti-DEPTOR. Whole cell extracts (20 μg) were used for Western blot analysis with primary antibodies against IL-1β (Abcam), NLRP3 (AdipoGen), NF-κB p65 (CST), Flag (Sigma), caspase-1, ASC, Gfi1 and β-Actin (Santa Cruz). 2.9 Measurement of cytokine production. BMDMs were seeded in 96-well plates and cultured overnight Nlrp3 −/ − mice also (Millipore) and blotted with rabbit anti-mouse caspase-1 antibody (Santa Cruz Biotechnology, Inc.). Secondary antibody was goat anti-rabbit HRP (Jackson ImmunoResearch Laboratories, Inc.). The membrane was exposed on radiographical film using SuperSignal West Pico kit (Thermo Fisher Scientific)..

BMDMs were incubated with a primary rabbit anti-NLRP3 polyclonal antibody (Santa Cruz Biotechnologies). Binding of the primary antibody was visualized by the addition of Alexa Fluor 594-conjugated anti-rabbit antibody (Invitrogen), and DNA was stained by the addition of 4,6′-diamidino-2-phenylindole (DAPI) dilactate Inspiration of a high concentration of oxygen, a therapy for acute lung injury (ALI), could unexpectedly lead to reactive oxygen species (ROS) production and hyperoxia-induced acute lung injury (HA..

Activation of Platelet NLRP3 Inflammasome in Crohn's

Applications. Species. Liu F, Liu T, Sun M et al. Maxing Shigan Decoction Mitigates Mycoplasma pneumonia-Induced Pyroptosis in A549 Cells via the NLRP3 Inflammasome Infection and drug resistance Mar 3 2021 [PMID: 33688221] (WB, Human) WB. Human. Reviews for GSDMDC1 Antibody (NBP2-80427) (0) There are no reviews for GSDMDC1 Antibody (NBP2-80427. The NLRP3 inflammasome is a key regulatory molecule of the inflammatory response. Its role in COPD is unclear. We investigated the NLRP3 inflammasome status in: 1) lung tissue samples from 38 patients with stable anti-caspase-1 sc-515 antibody (Santa Cruz Biotechnology, Dallas, TX, USA), which detects the fragment bated with anti-pro-IL-1 or actin antibody (Santa Cruz Biotechnology, Inc.) for 18 h at 4°C (8). Membranes were washed and incubated with donkey anti-rabbit immunoglobulin (1:1,000 dilution; GE Healthcare) conjugated to horseradish peroxidase. The Western blots were developed by using chemiluminescence and analyzed by digital densitometr (G and H) Nlrp3 +/+ and nlrp3 −/ (Santa Cruz Biotechnology, sc-2004) and HRP-conjugated anti-mouse secondary antibody (Santa Cruz Biotechnology, sc-2005). The blots were visualized using the ECL system (Animal Genetics, LR 01-01). For immunoprecipitation, 200 µg of whole cell lysates, cytosolic and mitochondrial fractions were incubated.

Cryopyrin Antibody (6F12) | SCBT - Santa Cruz BiotechnologyAscs | Bioz | Ratings For Life-Science Research

ACCEPTED MANUSCRIPT An optimized whole blood assay measuring expression and activity of NLRP3, NLRC4 and AIM2 inflammasomes Lev Grinstein 1, Hella Luksch , Felix Schulze1, Avril A.B. Robertson2, Matthew A. Cooper2, Angela Rösen- Wolff 1, Stefan Winkler # 1Department of Pediatrics, University Hospital Carl Gustav Carus, Technische Universität Dresden NLR family, pyrin domain containing 3 (NLRP3), controls the activity of inflammatory caspase-1 by forming inflammasomes, which leads to cleavage of the procytokines IL-1β and IL-18. Recent studies have shown associations of human NLRP3 polymorphisms with susceptibility to various inflammatory diseases; however, the association with allergic diseases remains unclear GSDMD antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC),Enzyme-linked Immunosorbent Assay (ELISA) applications. This antibody reacts with human samples. Cat.No. 20770-1-AP. KD/KO Validated

Histone-H3 antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC), ChImmunoprecipitation (IP),Enzyme-linked Immunosorbent Assay (ELISA) applications. This antibody reacts with human, mouse, rat samples. Cat.No. 17168-1-AP Immunogen affinity purified. Primary antibody notes. Post-translational regulation of Rho activity has been shown specifically for RhoA. This Rho protein is phosphorylated in vitro on serine 188 by cAMP- and cGMP-dependent kinases (PKA and PKG). Ser188 phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation

Cells were incubated with LC3B antibody (Life Technologies, Grand Island, New York) in antibody dilution buffer overnight at 4°C. Cells were washed in PBS (3×) and incubated with Alexa Fluor 488 conjugated anti-rabbit IgG secondary antibody (Santa Cruz Biotechnology, Dallas, Texas) for 4 h followed by washing Incubate membrane with Anti-rabbit IgG, HRP-linked Antibody (#7074 at 1:2000) and anti-biotin, HRP-linked Antibody (#7075 at 1:1000-1:3000) to detect biotinylated protein markers in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature Primary antibodies include IL-15 (Santa Cruz Biotechnology, sc-8437) and IL-15Rα (R&D Systems, AF247). All samples were incubated for 1 hour at room temperature with the appropriate Alexa Fluor-conjugated and high-cross-absorbed secondary antibodies in 5% donkey serum/0.01% Tween/PBS blocking solution (1:500 dilution) Global antibody supplier and research reagent supplier to the life science community. Find antibodies and reagents all backed by our Guarantee+

Gene silencing of non-obese diabetic receptor family

Antibodies, reagents, and resources, such as pathways, protocols & videos, to help you accelerate your research. Neurodegeneration. Hallmarks of Neurodegeneration and Cell Markers. Learn about key cellular mechanisms that drive neurodegenerative diseases. Epigenetics. Cancer Epigenetic Guide. Anti-mIL-10R1 antibody (1B1.3A) and anti-mIL-10 antibody (JES5.2A5) were obtained from Bio X Cell for the in vivo neutralization experiments. Commercially available polyclonal Grp78 antibody sc-1050 (Santa Cruz Biotechnology) was used to detect murine Grp78

NLRP3 inflammasome activation by mitochondrial ROS in

A 1-10 μg/ml concentration should be used when using thie antibody in an ELISA application. How to get rid of background when using Product F7465, ANTI-FLAG ® from rabbit,? To reduce non-specific background on tissue staining, a small amount of Triton X-100 (Product No. X100 or T8787), can be added to the wash buffers and antibody incubation. Abcam, the leading supplier of protein research tools to life scientists. Discover more from a range of 118,000 antibodies, kits, proteins and other reagent Bio-Techne's Mission is to build Epic Tools for Epic Science. We have a creative, caring team of colleagues throughout the world who bring unique perspectives and talents in support of that Mission and who embody our four key Values -- Empowerment, Passion, Innovation and Collaboration More>> This Anti-Caspase 1 Antibody is validated for use in IF, IP, WB for the detection of Caspase 1. Less<< Anti-Caspase 1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents After stimulation, cells were fixed in 2% paraformaldehyde and subsequently permeabilized in 0.25% Triton X-100 in PBS. For staining, cells were incubated with anti-NF-κB p65 antibody (Santa Cruz) in 0.1% Triton X-100 containing 2% goat serum in PBS. Secondary antibodies were anti-rabbit IgG, ads-FITC (Southern Biotech)

Assembly of the NLRP3/ASC inflammasome requires HOIL-1L

Knockdown was confirmed with Western blotting using whole cell lysates. siPrx1 (sc-36177) and scramble negative control (sc-37007) were obtained from Santa Cruz Biotechnology: siE6AP (sense), 5′-GCCCAGACACAGAA AGGUUTT-3′; scramble negative control (siCont-1, sense), 5′-UUGCGGGUCUAAUCACCGATT-3′ Toxoplasma gondii is a parasite that primarily infects through the oral route. Nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) play crucial roles in the immune responses generated during parasitic infection and also drive the inflammatory response against invading parasites. However, little is known about the regulation of NLRs and inflammasome activation in T. gondii. GSDMDC1 (64-Y) Antibody Santa Cruz Biotechnology sc . Labome.com DA: 14 PA: 47 MOZ Rank: 69. Gutierrez K, Davis M, Daniels B, Olsen T, Ralli Jain P, Tait S, et al.MLKL Activation Triggers NLRP3-Mediated Processing and Release of IL-1β Independently of Gasdermin-D; 2017;198:2156-2164 pubmed publisher; Rabbit Monoclonal Anti Cd25 Santa Cruz.

CSTP1 Gene - GeneCards | CSTP1 PseudogeneNLRP3 inflammasome pathwayFRG1FP Gene - GeneCards | FRG1FP PseudogeneSLC25A5-AS1 Gene - GeneCards | SLC25A5-AS1 RNA Gene

IL-6 Antibody (H-183) Santa Cruz Biotechnology, sc-7920 Santa Cruz Biotechnology Cat# sc-7920, RRID:AB_2127745 rabbit polyclonal 1:200 Endocrinology 2013 154: 3130-3140 10.1210/en.2013-1218 RRID:AB_2127745 phospho-STAT-3 p-Stat3 Antibody (B-7) Santa Cruz Biotechnology, sc805 NLRP3 protein level was inversely correlated with E-cadherin whereas it positively was correlated with IL 1β, active NF κB, N cadherin, vimentin, and MMP 9.This study for the first time showed that activation of NLRP3 inflammasome contributed to the progression of CRC and is correlated with the EMT process The NLRP3 inflammasome is dispensable for ER stress-induced pancreatic β-cell damage in Akita mice Accepted Manuscript The NLRP3 inflammasome is dispensable for ER stress-induced pancreatic β-cell damage in Akita mice Jie Wang, Mi-Young Song, Joo Yo.. The antibody of STAT3, p-STAT3, RORγt, IκBα, p-IκBα, p65, p-p65, NLRP3, ASC, IL-1β, caspase-1, or GAPDH was incubated at 4°C with the membrane overnight. After washing three times, the membrane was incubated with horseradish peroxidase-conjugated anti-rabbit IgG or anti-mouse IgG for 2 h at room temperature De Filippis et al. Molecular Brain (2016) 9:51 DOI 10.1186/s13041-016-0221-7 RESEARCH Open Access Ethanol-mediated activation of the NLRP3 inflammasome in iPS cells and iPS cells-derived neural progenitor cells Lidia De Filippis1,2*, Apoorva Halikere1,2, Heather McGowan1,2, Jennifer C. Moore3,4, Jay A. Tischfield3,4, Ronald P. Hart4,5 and Zhiping P. Pang1,2* Abstract Background: Alcohol abuse. formation of the NLRP3 inflammasome, caspase activity, and release of interleukin-1b. Furthermore, the SPA4 peptide treatment reduced the secreted levels of interleukin-1b from cells overexpressing Toll-like receptor-4 compared with cells expressing the dominant-negative form of Toll-like receptor-4. Together our results suggest that the SPA4.